Cobalt Chelating Superflow 4

Cobalt Chelating Superflow 4 is an immobilized metal affinity chromatography (IMAC) resin that separates proteins via their differences in affinity to cobalt. This product is recommended as a drop-in replacement for other tetra-dentate chelators and clarified feed streams.

Cobalt Chelating Superflow 4 is generated from a proprietary tetra-dentate chelating chemistry that has been optimized to provide increased flow rates (> 30 ml/min) and Protein binding capacities (> 60 mg protein per ml resin). Along with no metal leakage properties, this resin is ideal for either large batch or column purification.

Cleanable, rechargeable, and industrial process-ready, the Cobalt Chelating Superflow 4 resin is manufactured under cGMP and ISO 9001:2015 conditions. Regulatory Support File are available as well as DMF with USFDA

The product is shipped pre-charged and ready to use. Optimizing the separation usually requires some method development.

$488.00

Product Code: 37409SF4

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Table 1. Resin Characteristics

Bead Material Agarose
Bead Percentage 4%
Bead Size60-160 µm
Flow Rate1>30 mL/min (>1020 cm/hr) @ 25oC
AT3 Binding >6 mg/mL
pH Stability22-14
Storage Temperature2-8o C
Storage Buffer 20% Ethanol
FormSlurry
Chemical Stability2Avoid EDTA, EGTA, DTT, as well as other reducing agents and metal chelators. The use of primary amines (Tris, glycine, etc.) can weaken binding affinity and should not be utilized. Reactions in which detergents like Guanidine HCl (<6M) and Urea (<8M) are incorporated can be used sparingly. For the use of detergents and other additives, please consult the literature.
Physical Stability2Negligible volume variation due to changes in pH or ionic strength.

1Linear flow rate = volumetric flow rate (cm3/h)/column cross-sectional area (cm2)

2Data refer to the coupled product, provided that the ligand can withstand the pH or chemical environment. Please note the following: pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration and cleaning procedures.

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