Protein A Superflow 4

Protein A is a well-characterized antibody-binding protein composed of five homologous immunoglobulin-binding domains. Protein A binds strongly within the Fc region of several immunoglobulins and weakly to the Fab region of the VH3 class of immunoglobulins.
Protein A resins are the most frequently used affinity chromatographic media and are a standard technique for the production of monoclonal antibodies. Sterogene’s Protein A Superflow 4 resin is alkali stable, has a high static binding capacity (50 mg/mL), has a high flow rate (>1020 cm/hr), and can be used multiple times without losing activity.

Product Code: 937609SF4

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Table 1. Resin Characteristics

Bead Material Agarose
Bead Percentage 4%
Bead Size60-160 µm
Flow Rate1>30 mL/min (>1020 cm/hr) @ 25oC
Binding Capacity (static)> 50mg/mL
pH Stability3-13
Storage Temperature2-8o C
Storage Buffer 20% Ethanol
FormSlurry
Chemical Stability2Stable in all commonly used aqueous solutions and buffers.
Physical Stability2Negligible volume variation due to changes in pH or ionic strength.

1Linear flow rate = volumetric flow rate (cm3/h)/column cross-sectional area (cm2)

2Data refer to the coupled product, provided that the ligand can withstand the pH or chemical environment. Please note the following: pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration and cleaning procedures.

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